SOFMER / SFETD
Le 14/10/2021 de 09:00 à 11:00
Une analyse de la méthylation de l’ADN du génome entier chez des patients souffrant de douleurs chroniques de type nociceptive et neuropathique révèle des altérations de la méthylation de gènes impliqués dans le système neuro-musculo-squelettique.
Joane Le Carré (Sion, Suisse), Ludwig Stenz (Genève, Suisse), François Luthi (Sion, Suisse), Philippe Vuistiner (Sion, Suisse), Cyrille Burrus (Sion, Suisse), Ariane Paoloni-Giacobino (Genève, Suisse), Bertrand Léger (Sion, Suisse)
Objective : Nociceptive pain involves the activation of nociceptors without damage to the nervous system, whereas neuropathic pain is related to an alteration in the central or peripheral nervous system. Chronic pain itself and the transition from acute to chronic pain may be epigenetically controlled.
Material / Patients and Methods : In this study, a genome-wide DNA methylation analysis was performed using the blood DNA reduced representation bisulfite sequencing (RRBS) technique. Three cohorts including 20 healthy controls (CTL), 18 patients with chronic nociceptive pain (NOCI), and 19 patients with chronic neuropathic pain (NEURO) were compared at both the single CpG and differentially methylated region (DMR) levels.
Results : Genes with DMRs seen in the NOCI and NEURO groups belonged to the neuro-musculoskeletal system and differed between the NOCI and NEURO groups. Five genes, PNPLA6, RAB10, LRRC59, BMP1, and P3H3, had similar differences when comparing DMRs between the NOCI and CTL and NOCI and NEURO groups. In the NOCI group, DMRs were observed in genes particularly relevant to pain: RAB10, which encodes a protein that interacts with the δ-opioid receptor, and CGRP, which mediates nociceptive pain. In the NEURO group, DMRs were observed in MAGI2, a gene coding for a synaptic protein involved in GABAergic transmission, and in PVALB, a gene coding for a calcium-binding protein expressed in GABAergic interneurons.
Discussion - Conclusion : This study presents biological findings that help to characterize NOCI- and NEURO-affected pathways and opens the possibility of developing epigenetic diagnostic assays. This shows the important and specific effect of two types of chronic pain on the human epigenome.
Keywords : DNA methylation, chronic pain, biomarkers, neuropathic pain; nociceptive pain